Characterize membrane proteins in complex environments with confidence.
Membrane proteins are among the most challenging targets in drug discovery, yet they represent the largest class of therapeutic targets in the human genome. NanoTemper’s biophysical tools give you the stability and binding data you need to study these difficult proteins — in detergents, nanodiscs, SMALPs, or any other membrane-mimetic system.
Work directly with membrane proteins in their solubilized or membrane-mimetic environments, with no modifications and no compromises.
Characterizing membrane proteins starts with extracting them from their native lipid bilayer, process that can destabilize structure and compromise function. NanoTemper’s nanoDSF technology measures thermal stability using the intrinsic fluorescence of your protein’s tryptophan residues, requiring no dyes and working in any buffer system, including those containing detergents. This makes it possible to rapidly screen detergent conditions to find the one that best preserves your target’s stability. For binding studies, use Spectral Shift to measure interactions in solution, collecting high quality data whether your membrane protein is detergent-solubilized, reconstituted into lipid nanodiscs, or embedded in SMALPs.
From detergent screening to drug binding, NanoTemper supports every step in membrane protein characterization.
Membrane proteins demand methods that can handle their complexity: fragile solubilization conditions, low expression yields, and difficult-to-maintain functionality. NanoTemper’s tools are built to work with these targets as they are, not as you wish they were.
Two platforms. One workflow. Built for the most challenging membrane protein targets.
Stability Characterization with Prometheus Panta
Using nanoDSF and backreflection, Prometheus Panta measures the thermal stability of membrane proteins via intrinsic fluorescence. No labels, no dyes, and broad compatibility. Screen solubilization conditions, identify stabilizing ligands, and assess sample homogeneity before downstream experiments.
Affinity Measurement with Dianthus
Dianthus measures binding interactions with membrane protein targets in solution, with no immobilization required. Spectral Shift and TRIC technologies work effectively with detergent-solubilized proteins and nanodisc-reconstituted receptors, making Dianthus ideal for ligand screening and hit validation against GPCRs, ion channels, and transporters.
Together: A Complete Characterization Workflow
Prometheus Panta and Dianthus complement each other across the membrane protein characterization workflow. Panta confirms your target is stable and well-folded under your chosen conditions, while Dianthus measures how your compounds bind to it. Together, they give you the confidence to move forward with the right target preparation and the right candidates.
“NanoTemper helps us turn challenging biophysical tasks into routine workflows. Their intuitive solutions give us reliable data faster, so our teams can focus on advancing drug candidates.”
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