Monolith Omni
More answers.
Less complexity.
In-solution interaction profiling across challenging biomolecular interactions. Combine affinity, kinetics, and stability in a single workflow to characterize targets across modalities, from small molecules and nucleic acids to molecular glues and receptors, even when material is limited.
Affinity, kinetics, stability. One workflow.
Affinity, kinetics, stability. One workflow.
Mechanistic insight, without wasting precious sample.
Monolith Omni is designed to help you get more from limited material. Generate affinity data in minutes and expand beyond binding strength alone by combining affinity, kinetics, and stability in a single workflow.
Measure in solution, the way biology actually works.
By characterizing interactions free, in solution and without immobilization, Monolith Omni helps reduce surface-related artifacts and supports more biologically-relevant measurements across challenging samples and matrices.
Build a more complete interaction profile.
Affinity alone rarely tells the full story. Monolith Omni brings together complementary readouts to measure binding strength, add kinetic context, and assess qualitative stability, so you can interpret interactions with greater confidence.
Productive from the start.
Intuitive software and guided setup help teams get up and running quickly, from first assay planning through to clear, actionable data.
Get broader interaction insight with confidence, even when your sample is limited and your target is challenging.
technology tag
Prometheus™ Panta is powered by our unique technologie
DLS enables you to monitor the colloidal stability of your sample. This information helps you optimize your sample by making changes to the sequence or buffer environment, and measuring how those changes impact colloidal stability.
Your challenging targets, finally measurable.
Monolith Omni enables in-solution interaction characterization in a fluidics-free workflow that is simple to run and easy to maintain. By combining affinity and kinetic analysis in biologically relevant conditions, it helps reduce assay complexity while supporting challenging interactions across diverse modalities. With broad kinetic coverage, low operating burden, and high ease of use, Monolith Omni is designed to deliver richer interaction insight without adding workflow overhead.
Multi-parameter characterization. One platform. Free in solution.
Multi-parameter characterization. One platform. Free in solution.
Multiple insights.
Spectral Shift
Isothermally detects direct target engagement, independent of the binding site by measuring shifts in the emission spectrum of a fluorescently labeled target. Derives affinity constants from the ratiometric signal with sub-nanometer sensitivity that catches the binders other platforms miss.
nano Temperature Alteration Kinetics (nanoTAK)
Uses a precise laser-induced temperature jump to perturb the binding equilibrium. Relaxation to the new equilibrium is monitored in real via Spectral Shift. A global model fit yields rate constants in-solution, delivering insights into molecular binding kinetics.
nano Laser Induced Stability Analysis (nanoLISA)
nanoLISA detects unfolding and early aggregation by heating samples up to 95°C while monitoring spectral shifts. Unfolding alters the fluorophore’s environment, producing a clear sigmoidal curve. Ligand binding shifts this curve, showing stabilization through covalent and non-covalent interactions. Comparing thermal melting profiles with and without ligand enables orthogonal hit confirmation and deeper insight into binding.
Temperature Related Intensity Change (TRIC)
Measures fluorescence changes after a brief temperature increase. Confirms hits with an orthogonal signal and reveals ligand-induced effects like aggregation behavior.
“NanoTemper helps us turn challenging biophysical tasks into routine workflows. Their intuitive solutions give us reliable data faster, so our teams can focus on advancing drug candidates.”
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More about Monolith.
Complete your setup.
Simple setup.
Consistent performance.
Monolith Omni uses a capillary-based workflow designed to keep sample handling straightforward. High-quality capillaries support reproducible measurements, helping you generate reliable interaction and qualitative stability insight with less preparation overhead.
Software that's easy to use and easy to learn.
Getting started with affinity measurement is simple with Monolith software. Control software is designed for easy, customizable set-up of experiments and allows you to view data acquisition in real time. Powerful Analysis software enables you to sort, highlight, and get insight into aggregation. All software has a clean interface and intuitive user experience, so you’re ready to get started as soon as your Monolith fires up.
Maximize instrument performance.
Without the Downtime .
NanoTemper instrumentation is built to the highest standards of quality and reliability; however, it’s important to keep your instruments in good working order to ensure accurate, high-quality data output. Don’t let unexpected delays slow you down or interrupt your lab workflows. Service offerings include: relocation services, operational qualification procedures, and full coverage repair and validations.
FAQs
Monolith
Monolith Omni combines affinity, kinetics, and qualitative stability in a single workflow. This helps researchers build a broader interaction profile by measuring binding strength, adding kinetic context, and assessing ligand-induced stability effects from the same experimental workflow.
Monolith Omni builds on NanoTemper’s proven Monolith platform by extending beyond affinity measurement alone. In addition to in-solution affinity analysis, it adds kinetic and qualitative stability insight to help researchers interpret molecular interactions more completely.
Monolith Omni brings multiple interaction parameters together in a single experimental workflow, reducing the need to switch between separate methods for affinity, kinetic, and stability-related insight. This helps lower workflow complexity, preserve sample, and simplify interpretation.
Monolith Omni is designed for rich, multi-parameter interaction characterization rather than high-throughput screening. It is best suited for applications where depth of insight, limited material use, and simplified workflow matter more than maximum screening scale.
Monolith Omni is a strong fit when affinity alone is not enough and you need additional kinetic and qualitative stability context without adding multiple separate assays. It is especially valuable for challenging targets, diverse molecular modalities, and experiments where sample is limited.