Data analysis is a challenging process—researchers have mountains of data to go through—and expediting analysis with automated software solutions is a crucial tool to save you time and effort. However, no algorithm can replace the human eye when it comes to double-checking the results. So how can researchers save time with data processing but also feel confident in their conclusions?
PR.Stability Analysis software has a great new feature—Region of Interest—to help you develop that much-needed confidence in your conclusions. This feature enables you to focus only on the portion of the data that is important to you, and the fitting algorithms are re-tooled only for the portion of the data you highlight. Catch subtle transitions, quickly view and rank the parameters of interest important to you, or focus only on a specific domain of your protein. Welcome to another installment of Application Academy: How to Use Regions of Interest.
Until now, Prometheus users processing data using PR.ThermControl or PR.ChemControl software have been applying the inflection point (IP) and onset algorithms to their full data set across the complete temperature or chemical gradient. But many proteins do not exhibit unfolding until quite a way through the gradient. There can be issues with sloping baselines that convolute analysis. Perhaps bubbles sneak into your sample. Or maybe you only use one domain transition to draw conclusions about your experiments. The region of interest feature allows you to select only a portion of the gradient to analyze so you can determine true thermodynamic parameters from your data. Improve your data analysis while focusing only on the information you care about.
Check out this video with NanoTemper Application Specialist Lindsay Dawson to see how easy it is to define and analyze a region of interest in your dataset.
PR.Stability Analysis is designed to enable our Prometheus users to get highly precise information about the data that is most important to them. To get even more out of your Prometheus, sign up for the NanoTemper Explorer Community and chat with other NanoTemper users. Plus, you can get your questions answered from our team of dedicated Applications Specialists. You can also find great tips on PR.Stability Analysis software, various protocols, and even fun facts! Comment to let us know what other topics you’d like to see, and stay tuned for additional tips and tricks on PR.Stability Analysis and more in our Analysis Academy series.