Measuring with precision matters when the differences between your candidates are subtle. Prometheus delivers the high-resolution stability data you need to spot those differences and decide which candidates are best to work with moving forward.
With multiple technologies collecting conformational and colloidal stability data from a single sample, you gain a deeper understanding of your molecule’s behavior when assessing your protein. Prometheus offers a combinatorial approach to selecting ideal drug candidates and conditions.
Prometheus works for large experiments with a single purpose, such as buffer screening; or for complex, multi-step processes like developability profiling. And when big projects come your way, get all your conformational and colloidal stability info in a hands-free, automated manner.
It doesn’t take an expert to learn everything you need to know about your protein samples. Prometheus makes it easy to get started, both with operation and data analysis. Spend less time struggling to interpret your data and more time making decisions about your proteins.
Create biologics developability profiles during early-stage screening.
Perform comparability studies to ensure that changes to manufacturing processes or sites don’t affect the drug product.
Use combinatorial stability parameters to find the optimal storage buffers for your drug candidates.
Create biologics developability profiles during early-stage screening.
Differentiate AAV serotypes and determine the purity of your vector preps
Maximize protein solubility and stability before crystallization or Cryo-EM to improve the quality of your structural data
Screen for small molecule drug candidate compounds that increase the stability of your target protein, indicating an interaction
Determine if a protein is more likely to self-associate, and therefore become viscous or aggregated
Extrapolate the long-term stability of your proteins with high-temp isothermal measurements
There are several versions of Prometheus available for whatever your lab needs. They all provide the highest-resolution, highest-quality data, and do so on an intuitive and user-friendly software interface.
Find the right configuration for your lab.
Choose Prometheus Panta for full stability and developability profiles for your proteins, including thermal stability, particle sizing and purity, and aggregation
For high-throughput developability or formulations screening, turn to the Prometheus Panta with the Robotic Autosampler to get full stability profiles on up to 1536 samples, hands-free
Prometheus uses a number of technologies to characterize thermal unfolding, particle sizing, and aggregation. Each one tells a different story about stability, and is used for a variety of applications. But they have one thing in common — they are all label-free.
For monitoring thermal stability during biologics formulation, developability, production, and manufacturing; evaluating target-ligand complexes before affinity-based screening; or optimizing conditions for structural biology conditions
It measures thermal or chemical unfolding label-free, under native conditions by detecting changes in a protein’s intrinsic fluorescence during a thermal ramp or in the presence of a chemical denaturant
For screening recombinant proteins expressed from different constructs, biologics formulation screening, sample optimization for biophysical assays, and characterization of self-interactions
It determines the size of the molecules in your sample prep, and whether or not there are multiple sample sizes present
For determining if complexes have formed, measuring how much genetic material a viral vector contains, or extrapolating how proteins will interact with themselves at high concentrations.
It looks at the overall average scattering intensity of your solution and correlates that to the molecular weight of your particles
It indicates the presence of large aggregates by measuring light intensity loss due to scattering, i.e., turbidity
It determines aggregation status by measuring light intensity loss due to scattering
Prometheus provides a wealth of information about the stability of your proteins. Many of these parameters are collected concurrently, without the need for additional experimental set-up. Get the most information out of your protein samples. Learn more about which parameters you get from Prometheus.
Polydispersity index represents distribution of size populations
Low PDI values indicate prep is free of large aggregates or multiple protein populations
Melting temperature, or point at which 50% is unfolded
Ranking candidates by melting temperature is a well-established method for finding most thermostable constructs, considered better selections for further experimentation
Temperature at which thermal unfolding begins, measured from fluorescence ratio
More thermostable proteins have higher onset of unfolding; furthermore, proteins with Tonset values close to their Tms are more uniformly folded and therefore more stable
Activation energy of unfolding (*parameter derived from data)
The more energy required to drive unfolding of a protein, the more stable it is
Point at which irreversibility happens when unfolding, thereby making aggregation more likely
Engineer or formulate proteins to avoid or delay irreversible unfolding and loss of function
Hydrodynamic radius shows size of particle in solvated state
Establishes baseline sizing parameter about a protein of interest, used to compare before and after changes to formulation buffer or production processes
Onset temperature of turbidity, or large aggregates >12.5 nm radius
Large, amorphous aggregates contaminate preps and lower safety, activity, and stability of a prep; aim for high Tturbidity values or no change in turbidity signal
Temperature at which scattering intensity begins to change, indicating increased size and aggregation
Additional parameter for evaluating colloidal and conformational destabilization of a protein; higher Tscattering means a more stable protein
Second virial coefficient used to extrapolate self-association behavior at higher concentrations
Negative B22 values indicate propensity towards self-interaction, and therefore greater likelihood of aggregating at the high concentrations required for clinical use
Diffusion interaction identifies onset of unfolding and impact on colloidal stability
Negative kD values indicate propensity towards self-interaction, and therefore greater likelihood of aggregating at the high concentrations required for clinical use
The concentration of a denaturant that causes 50% of proteins to unfold
Higher Cms indicate a more stable protein
The Gibbs free energy of protein unfolding, a thermodynamic measure of the likelihood a folding event may occur
Proteins with more negative ΔG are more likely to fold. Changes in the Gibbs free energy are measured with ΔΔG, which shows the relative stability of a protein at various concentrations or conditions.
Prometheus isn’t the only instrument around that provides the data you need for your work. So why choose it? Prometheus offers the highest-quality data so you never doubt the quality of your measurements, and make decisions about your proteins with confidence.
Provide replicate measurements with minimal deviation between individual samples
Detect multiple unfolding events or distinguish differences in unfolding profiles even among proteins with similar Tms
Measure samples at high or low concentrations
See meaningful transitions even at low sample concentrations
Distinguish the signal of your protein of interest from the signal of the buffer or matrix, without the need for a blank or calibration
Getting started with protein stability experiments is simple with Prometheus software. Control software is designed for easy set-up of experiments and allows you to view data acquisition in real time. And powerful Analysis software enables you to sort, highlight, and rank the parameters that tell you about your proteins of interest. All software has a clean interface and intuitive user experience, so you’re ready to get started as soon as your Prometheus fires up.
All you need for Prometheus experiments are capillaries. Prometheus capillaries are manufactured using the same stringent protocols used for diagnostic-grade materials to ensure the best results. They come individually or in chips spaced for use with 384-well plates.