Developing good scientific research is a long process. You start with a hypothesis, conduct experiments to support it, and draw conclusions from there. But the process is iterative—every question answered spawns a whole new set of questions. As researchers work to approach sound, supported conclusions, it is important to be able to compare how your ideas have evolved.
PR.Stability Analysis software offers a great new feature—Percent Similarity— that is used to analyze how your formulations and preparations compare to earlier experiments. It enables you to get quantifiable data that compares an experimental run to a reference. Whether you’re creating new buffer formulations, developing biosimilars, or comparing mutants of a protein of interest, Percent Similarity is a fast and easy way to evaluate how your changes have affected unfolding compared to your reference sample. Welcome to the next installment of Application Academy: using Percent Similarity.
Prometheus users have long been using their data to study how changes to their protein environment affect the stability of their formulations. Until now, the fastest way to do this was to export all the data and rank the shift in Tms manually. But PR.Stability Analysis offers solutions to dramatically speed up this process. Not only is it easier to prioritize your data (see: Regions of Interest), but now you can get a rapid quantitative readout of how your experimental data compares to a reference sample of interest.
Use the “assign reference” feature to select which run is your control, and then see how the rest of your experimental data compares by percent similarity to the unfolding curve. You’ll get a fast summary of which formulations are the most similar, and which cause the most dramatic change in your protein’s stability profile.
Watch the video below with NanoTemper’s Application Specialist Lindsay Dawson, where she explains how to apply these features to your data analysis.
Consistency is key to getting great results. Using the percent similarity feature in PR.Stability Analysis software to compare your samples to a known or defined reference will help you to troubleshoot and confirm that you are getting consistent results. But software isn’t the only way to boost output—join NanoTemper’s Explorer Community today and gain access to many essential resources. Meet and share information with other Prometheus users, find protocols and tips online, or connect with our team of expert Applications Specialists. And keep looking out for new installments in Application Academy to learn more about how to get the most out of your data.