How to navigate your Prometheus data in PR.Stability Analysis software

One of the challenges many scientists face is how to best present their data. No matter how stunning and ground-breaking your conclusions are, you’re not going to convince anyone your hypothesis holds true unless you can communicate and present it in the best way possible. Researchers aren’t trained in graphic design and marketing, yet presenting data effectively requires both. So how does a humble scientist put on these additional hats to convince people their work is valuable and important?

PR.Stability Analysis software does a lot of the hard work for you! Selecting the data you want to display and present, and how you want to present it, is simple and easy, saving you time while giving you concise-looking charts and graphs for sharing your results with others. This is the first installment of a series of pieces designed to help you get the most out of your Prometheus data. Let’s start with a basic overview of what PR.Stability Analysis can do. Welcome to Display Options 101!

 

Why are display options important?

Presentation matters. Whether you’re presenting at a conference, publishing your data in a trade journal, or simply sharing data with your lab mates, presentation matters. No one will be convinced of your conclusions if they can’t understand your data, and the first step in comprehension is an effective presentation.

Prometheus users familiar with PR.ThermControl and PR.ChemControl software know how easy it is to see the inflection points and onsets of their melting data. The next step is more advanced data analysis and processing, and PR.Stability Analysis is the software to handle it. The software allows you to efficiently analyze your data from multiple runs at the same time.

 

What can I do with PR.Stability Analysis software?

  • View all your data together: PR.Stablity Analysis allows you to look at multiple data sets at once. You can upload multiple files, or analyze multiple runs from the same file, all in the same window.
  • Visualize your inflection points (IPs) and onsets: The ratio view, first derivative, and scattering curves can now all be displayed at the same time. This allows you to quickly scroll through your data sets and see both unfolding and aggregation information at the same time.
  • See variation in your replicates: With the auto-merge feature, your annotated replicates will be merged into a single curve, creating a graph that displays the average and the variation of your replicates. We will cover more about data merging in an upcoming tutorial.
  • Toggle displays to get the view you want: It is easy to alter the look and feel of your graphical data using the software. Remove gridlines, select whether you want to view markers, and choose whether to view ratio or single wavelength data using a useful sidebar.

 

How do I work the PR.Stability Analysis display options?

Check out this video with NanoTemper Application Specialist, Lindsay Dawson, to see how easy it is to display and alter your data presentation.

Here are the display options available to you:

  • Select either the ratio view or evaluate a single wavelength
  • Choose whether you want to display the first derivative or the scattering plot
  • Choose whether you want to display markers
  • For a cleaner presentation, you can remove the gridlines from your graphs

 

Improve your data presentations today with PR.Stability Analysis

PR.Stability Analysis software was designed to enable our Prometheus users to quickly process and present their data without having to rely on third party data analysis programs. Sign up for the NanoTemper Explorer Community to chat with other Prometheus and NanoTemper users and get your questions answered from our team of dedicated applications specialists. You can also find great tips on PR.Stability Analysis software, various protocols, and even fun facts!

About the Author

Stefanie Kall is an Applications Specialist with NanoTemper Technologies. She received a biochemistry PhD from University of Illinois in Chicago for her work characterizing inhibitors for kinases via X-ray crystallography. She also has a MSc in Biotechnology from Northwestern and a BSc from (The) Ohio State University in Biochemistry. When she is not training people on NanoTemper instruments, she enjoys running, baking, and playing video games.
Stefanie Kall