Two different algorithms are used to detect Aggregation from a TRIC-trace. In a Binding Check experiment or a Single-Dose Screening the fluctuations are quantified, occurring within the TRIC trace. For Binding Affinity analysis or Affinity Screening data, if possible, a multi-trace algorithm is applied that compares all TRIC-traces obtained for one ligand with one another and searches for significant deviations.
The aggregation quality is set to negative if the aggregation algorithms described above detect aggregation. Otherwise the aggregation quality is set to positive, meaning no aggregation was detected. The automatic setting of the aggregation quality can be overridden by the user as described in Quality Checks.