In Single-Dose Screening a Ligand is considered a non-binder if it yields results in TRIC measurements that are similar within a certain user-defined threshold to the Reference Sample. In Affinity Screening a ligand is considered a non-binder if the Dose Response Curve reaches less than 25% Saturation as determined by the fit (either Kd Fit Model or Hill Model ). In general, for Single- Dose Screening and Affinity Screening the Response Amplitude will determine if DI.Screening Analysis software considers a ligand a non- binder or assigns it a different Ligand Category.