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Dianthus NanoPedia Potential Hit

Potential Hit

Potential Hit is a Ligand Category in Single-Dose Screening and Affinity Screening. It is a sub-category of the Inconclusive ligand category. Potential hits are those that show either Ligand-Induced Photobleaching or a negative Ligand-Induced Fluorescence Change (i.e. Quenching). These hits are called potential hits because additional Specificity Test or Control Experiments might be necessary to distinguish a binding-induced effect on the data from a ligand-induced artifact. For the Ligand Autofluorescence category an additional control experiment may be necessary. Autofluorescence is its own main category because it has shown to be useful in case of Ligand libraries that contain red-fluorescent substances.

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