In the DI.Screening Analysis software Ligands that were measured in TRIC screenings are automatically categorized to select for Hits / Binders and to single out Non-Binders or ligands that cause artifacts, such as Aggregation of the Target molecule.
Another Ligand Category is the category „inconclusive“, which has several sub- categories. These are:
- Inhomogenous Response. A ligand is assigned this category if the determined signal area of replicates in a Single-Dose Screening deviate too much from the mean of the ligand areas. The relevant determining factor of the Quality Checks is the Inhomogeneity Quality.
- Potential Hit. A ligand is assigned this category if a ligand dependent decrease in Initial Fluorescence (i.e. Quenching) is observed. In this case only a Specificity Test can reveal if this ligand is a true hit.
- Not Reproducible. A ligand in a single dose screening is assigned this category if one of the Quality Checks returns different results for the measured replicates (e.g. one replicate showed aggregation whereas the other replicate did not).
- Scan Anomaly. A well is assigned this category if the Well-Scan Quality check shows an anomaly in one of the Well Scans.
The ligand category inconclusive is not to be confused with inconclusive results of Quality Checks.