Screening

TRIC can be used to test large numbers of interactions. Typically, a Ligand library of Small Molecules, Peptides, protein constructs or fragments (see Fragment Based Drug Discovery) is tested against one Target protein, for example a Kinases or receptor. There are two types of screenings:

• Single-Dose Screening: A primary screening for Hit-identification. It provides a simple yes/no answer as to whether ligands interact with the target or not. This helps to determine which ligands are worth a more in-depth investigation.
• Affinity Screening: Affinity characterization of ligands that showed an interaction in the primary screen. The obtained Dose Response Curve curves are then used to rank identified Binders for their Binding Affinity to the target.